Developing advanced organoid-based platforms for disease modeling, drug discovery, and alternative toxicity testing

• 인간 폐·혈액·근육·췌장 ·심장 ·피부 등 다양한 오가노이드 분화 기술개발
• 섬유화, 바이러스 및 세균 감염병 질환 모델링 구축 및 치료제 개발
• RNA 기반 항암제 개발
• 화학물질/환경유해인자 및 약물에 대한 폐 독성평가 /대체시험법 개발

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• HTRA2 alternative splicing 기반 암 억제 기전규명을 통한 새로운 RNA 조절 췌장암 치료제 개발 (과학기술정보통신부, '25~'28)
• 멀티 오간-시스테모이드 기반 임상 0상 안전성, 유효성 평가 플랫폼 개발 (과학기술정보통신부, '23~'27)
• 형광표지 도입 인간 폐 오가노이드 활용 생활화학제품 함유 혼합물의 실시간 폐독성 평가법 개발 (환경부, '22~'26)

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• 감염병 대응 첨단바이오의약 안전성 평가를 위한 인체 면역환경 모사 기반 비임상평가시스템 개발 (과기정통부 '24~'25)
• 심실형 심장오가노이드를 활용한 의약품 평가시스템 개발 (과학기술정보통신부, ‘22~’23)
• 첨단기술을 활용한 대체시험법 검증 기반 연구 (식품의약품안전처, '22~'23)
• 생활환경 유해물질 대체 친환경 신소재 개발 및 플랫폼 구축 (과학기술정보통신부, '21~'23)
• 인간 줄기세포 유래 장기유사체 모델 기반 독성 및 약물대사 측정표준 기술 개발 (산업통상자원부, ‘20~’23)
• 생체모사 모델 활용 나노기술 융합 항노화 첨단바이오 의약산업 고도화 기획연구 (대전시-대덕특구 융합확산 기획 및 창의융합 사업, '22)
• 폐 오가노이드를 이용한 결핵 치료제 평가모델 개발 (보건복지부, '21~'22)
• 3차원 장기(organ) 및 가상 장기 모델을 이용한 바이오의약품 안전성 평가기술 마련 연구 (식품의약품안전처, ‘21)
• 인간 폐 오가노이드를 활용한 폐섬유증 바이오마커 발굴 및 발병 조절기전 규명 (교육부, ‘18~’22)
• CT 영상 기술기반 환경성 폐질환 평가를 위한 줄기세포 유래 3D 폐 오가노이드 (미니장기) 모델 개발 (환경부, ‘18~’22)

• Seung-Yeon Kim, Ji-Won Baek, Eo Jin Kim, Sathiyaraj Srinivasan, Kee K. Kim, Eun-Mi Kim. CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoring. Materials Today Bio, 2026, 37:102972

• Seongmin Kim, Seri Choi, Seung-Yeon Kim, Ji-Won Baek, Chungoo Park, Eun-Mi Kim. Transcriptomic profiling in human lung organoids uncovers metallothionein response as a signature of PHMG-p-induced lung toxicity. Envrionmental Toxicology and Pharmacoloy. 2025,120;104852

• Seri Choi, Seung-Yeon Kim, Ji Won Baek, Won Keun Oh, Kee K Kim, Eun-Mi Kim. Human lung organoids reveal conserved CYP1A1 and metallothionein activation in response to particulate matter exposure. Ecotoxicology and Environmental Safety, 2025, 303:118909

• Haesoo Jung, Yong-Eun Kim, Eun-Mi Kim, Kee K Kim. Alternative splicing of CHI3L1 regulates protein secretion through conformational changes. Genes Genomics. 2025, 47(5):571-579.

• Jung-Min Lee, Kee K. Kim, and Eun-Mi Kim. Stress granule reporter system to assess perfluorooctanoic acid gastric toxicity in vitro. Molecular & Cellular Toxicology. 2025, 21; 701-708

• Nam- joon Cho, Seung-Yeon Kim, Sung-Gwon Lee, Chungoo Park, Sunkyung Choi, Eun-Mi Kim, and Kee K Kim. Alternative splicing of PBRM1 mediates resistance to PD-1 blockade therapy in renal cancer. THE EMBO JOURNAL. 2024, 43(22):5421-5444

• Sangsoo Lee, Seung-Yeon Kim, Eunhey Kwon, Sunkyung Choi, Da-Min Jung, Kee K. Kim, and Eun-Mi Kim. A novel G3BP1-GFP reporter human lung cell system enabling real-time monitoring of stress granule dynamics for in vitro lung toxicity assessment. Ecotoxicology and Environmental Safety. 2024, 269:115755

• Seung-Yeon Kim, Ji-Ae Choi, Seri Choi, Kee K Kim, Chang-Hwa Song, Eun-Mi Kim. Advances in an In vitro Tuberculosis infection Model using Human Lung Organoids for Host-Directed Therapies. PLos Pathogens. 2024, 20(7):e1012295

• Namjoon Cho, Da-Min Jung,Eun-Mi Kim and Kee K. Kim. Establishment of a stress granule reporter system for evaluating in vitro colon toxicity. Animal cells and systems. 2024, 28(1); 315-325

• Seongyea Jo, Sung Bum Park, Hyemin Kim, Ilkyun Im, Haneul Noh, Eun-Mi Kim, Ki Young Kim, Michael Oelgeschläger, Jong-Hoon Kim and Han-Jin Park. hiPSC-derived macrophages improve drug sensitivity and selectivity in a macrophage-incorporating organoid culture model. Biofabrication. 2024, 16(3);035021-035021

• Eunhye Kwon, Da-Min Jung, Kee K. Kim, Eun-Mi Kim. A new G3BP1-GFP reporter system for assessing skin toxicity by real-time monitoring of stress granules in vitro. Toxicology letters. 2024. 397;48-54

• Kyungtae Lim, Mi-Ok Lee, Jinwook Choi, Jung-Hyun Kim, Eun-Mi Kim, Chang Gyu Woo, Chaeuk Chung, Yong-Hee Cho, Seok-Ho Hong, Young-Jae Cho, Sun-Ju Ahn. Guidelines for Manufacturing and Application of Organoids: Lung. International Journal of Stem Cells. 2024, 17(2) 147-157

• Sangsoo Lee, Da-Min Jung, Kee K. Kim, and Eun-Mi Kim. Establishments of G3BP1-GFP stress granule monitoring system for real-time stress assessment in human neuroblastoma cells. CHEMOSPHERE. 2024, 36:142485

• Sangsoo Lee, Haesoo Jung, Sunkyung Choi, Namjoon Cho, Eun-Mi Kim & Kee Kwang Kim. Intron retention decreases METTL3 expression by inhibiting mRNA export to the cytoplasm. BMB Reports. 2023, 56(9) 514-519

• Ji-eun Kim, Eun-Mi Kim, Hyang-Ae Lee, Ki-Suk Kim. Effective derivation of ventricular cardiomyocytes from hPSCs using ascorbic acid-containing maturation medium. Animal cells and systems. 2023, 27(1); 83-93

• Sunkyung Choi, Namjoon Cho, Eun-Mi Kim and Kee K. Kim. The role of alternative pre-mRNA splicing in cancer progression. Cancer Cell International. 2023, 23(1) 249

• Namjoon Cho, Jaegeon Joo, Sunkyung Choi, Bu-Gyeong Kang, Andrew J. Lee, So-Yeon Youn , Su-Hyung Park, Eun-Mi Kim, Eliezer Masliah , Yuji Ko , Sun-Shin Cha, Inkyung Jung, Kee K. Kim. A novel splicing variant of DJ-1 in Parkinson's disease induces mitochondrial dysfunction. Heliyon, e14039

• Sunkyung Choi, Hyo Seong Lee, Namjoon Cho, Inyoung Kim, Seongmin Cheon, Chungoo Park, Eun-Mi Kim, Wantae Kim, and Kee K. Kim. RBFOX2-regulated TEAD1 alternative splicing plays a pivotal role in Hippo-YAP signaling. Nucleic Acids Research, 2022, 50(15):8658-8673

• Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-eun Kim, Dae-Hwan Nam, Gohar Manzar, Su-Man Kim, Hyung-Sik Kang, Kee K. Kim, Sang-Hun Jung, Ju-whan Lee, Minseob Lee, Han-Jin Park, Won Keun Oh, Chungoo Park, Chang Hyun Lee, Eun-Mi Kim. Pulmonary fibrosis model using micro-CT analyzable human PSC-derived alveolar organoids containing alveolar macrophage-like cells. Cell Biology and Toxicology, 2022. 38(4);557-575

• Seri Choi, Sunkyung Choi, Yeongsoo Choi, Namjoon Cho, Seung-Yeon Kim, Chang Hyun Lee, Kee K. Kim, and Eun-Mi Kim. Polyhexamethylene guanidine phosphate increases stress granule formation in human 3D lung organoids under respiratory syncytial virus infection. Ecotoxicology and Environmental Safety, 2022. 229:113094

• Sunkyung Choi, Eun-Mi Kim, Seung-Yeon Kim, Youngsoo Choi, Seri Choi, Nam-Jun Cho, Han-Jin Park, and Kee K. Kim. Particulate matter exposure exacerbates cellular damage by increasing stress granule formation in respiratory syncytial virus-infected human lung organoids. Environmental Pollution, 2022. 315:120439